Enzyme kinetics with Eisenthal & Cornish-Bowden
While recently going through some of the basics in enzymatic kinetics, I was asked to demonstrate some of the methods used to linearize the very well known Michaelis-Menten equation.
The most used linearization is the Lineweaver-Burk plot that plays with 1/v vs 1/s. But it was the Eisenthal & Cornish-Bowden method that just amazed me over it’s simplicity.
Even though I couldn’t find much about the method on any website, I did find the original published paper by none other than Eisenthal and Cornish-Bowden. And they couldn’t make it any simpler. Without going into details, it’s just a question of joining the dots and seeing where the lines crossover. You get Km and Vmax on the spot!
It’s amazing how these scientists made such a conclusion in 1973. It seems just too simple to be true. It even seems as if they had been playing around with graphs and they stumbled upon it.
Nonetheless, that was just my quick thoughts…
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True.I was also trying to figure it out.I wanted to find details as part of what I had to teach as part of the M.Sc.—Masters program in Mumbai India.I wish I could find a simple representation of the graph wish i could show the students.
[...] time back in October (2006) I was messing around with enzyme kinetics and was asked by one of my professors to look into the [...]